--start--- read the handout about adaptation to diseases we talked before about providing UVB light for reptiles and the proper spectra needed. the light should be about 12-24 inches away from the animal. it should not be on all day, either. UV lights do not last forever; after a period of months, the spectra they emit is not the same anymore. you have to replace those bulbs every four to six months. this is especially important for young, growing animals. the other area to get into is the issue of sexing of animals - people may ask the sex of the animal; this is important b/c some disease conditions are related to reproductive tract. snakes: there is a suggestion of sex by shape and length of tail caudal to cloaca. in males, hemipenes is in the tail, so tail base is thicker, wider, and tail is longer. more obvious when male and female are side by side. in boidae, there are vestigial spurs on either side of the cloaca, thought to be remnant of hind legs in those animals. that can sometimes cause trouble, b/c we think males have bigger spurs than females but that is really subjective. but if they are about 1/4 inch, probably male. for most breeders, if they need to know, they can do probing of theventral tail area - insert a smooth blunt probe into recesses of hemipenes, counting # of scale lengths - in males, probe should go in 6-18 scale lengths. in female, only 2-6. this should only be done if you know how to do it; owners shouldn't do it if they've never done it before. it can cause damage. turtles - tail is an important marker - male is often longer, wider at base. also the cloacal opening is more caudal in the male than female. in female, cloaca is about the level of the scute on the shell. in male, more caudal. in box turtles, iris color can be a hint. in males, tends to be deep red, females, brown/tan color. often males are smaller than females. in many tortoises, the bottom shell is concave, too, so that he can mount the female. in iguanas and other lizards, males are often larger than females and more colorful in breeding season. often in lizardsin general, the males have more body appendages, like Jackson's chameleon has these horns or helmets. may have spurs or other appendages. thinking of iguana, a large something fold. the ear coverings are largerin the male than the female. another aspect to look at anatomically is the femoral pores running along the medial surfaces of the leg, in femoral area. these are small or imperceptible in females, but large with hard waxy covering in males. immune system of these animals: we know that reptiles do have immunoglobulins; they usually make IgM and IgG. IgA, IgD, and IgE have not been seen complement system is not known; may occur, not yet found. we do know that reptiles, even though considered "poikilotherms" which base their temperature on the environmental temperature, can sometimes have pyrexic reactions to certain bacterial toxins. they can mount a febrile response, albeit only a degree or two, when exposed to bacterial toxins. we also know that in some spp of reptiles, they can develop agglutinating or neutralizing antibodies to bacteria and viral antigens. overall state of health and ambient temperature enhance immune response. the ability of the owner to provide a habitat and an environment that will promote the health of these animals is really important. gram negatives are the primary pathogens in most reptiles; by and large if you start culturing these animals you find gram negatives. gram positive infections are rare, but there are always exceptions. salmonella is a serious concern; the more we know about reptiles, the more problems we see. [end of serious note-taking. i'm feeling more like just listening, right now.] note: don't treat reptiles for salmonella - makes resistant organisms. reptiles just have it. use good husbandry to reduce shedding. don't let kids handle it. don't let mom nurse baby after handling reptile, without washing hands. do not let iguana walk on counter and then prepare baby bottle on it. mycotic disease usually presents as dermatitis, not internal dz. when mycotic organisms gain access to the body they do end up in lung, alimentary tract. these animals end up with long, slow demise no matter what you do sometimes, then at necropsy you find these granulomatous lesions in GI tract and lungs. do cultures for these. however, if you find an organism, that doesn't mean it is the pathogen. consider other possibilities. there may be other signs, other pathologic evidence. it's not always straightforward. histopath is important. if you section lesions and find good evidence of branching hyphae, repro stages of fungi, more likely to be involved in pathogenic process. dermatologic infections occur - saprophytes tend to be in the dirt, leaves, etc - if there is a wound, animal can easily pick up a fungus. we see crocodilia and chelonia with fungi more than lizards or snakes. with viruses, there is a growing list of them out there; we can't and won't go over all of them but one of the early ones - a herpes virus that was localized in pond turtles in california; other turtles have been found to have it as well. they all had primary hepatic necrosis. in snakes, the paramyxovirus and a retrovirus are two prime organisms causing disease. much attention is being given to it. [again, i'm sick of takingnotes already! ] ---end---